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以陕西省汉中市5个县的11个群体76份红豆杉样本为对象,基于15条ScoT引物分析红豆杉种质资源遗传多样性和群体结构,以期为汉中秦岭地区红豆杉的区域性保护和种质资源有效开发利用提供理论依据。结果表明:(1)位点水平上红豆杉遗传多样性较低(Na=1.96,Ne=1.49,H=0.30,I=0.46);(2)聚类分析将位点水平的红豆杉供试材料分为4组,与群体结构分析结果(K=4)基本一致;(3) 11个群体红豆杉遗传多样性较低(Na=1.52,Ne=1.30,H=0.18,I=0.37)且遗传变异主要来自群体内(79%);(4) 11个群体聚类为4组,略阳县郭镇(GZ)群体与留坝县4个群体聚为一组,勉县群体与佛坪县2个群体聚集为一组,与主坐标分析(PCoA)结果聚集一致。上述结果表明汉中秦岭地区红豆杉遗传多样性较为狭窄,建议对汉中秦岭地区红豆杉开展就地保护以及构建多资源种质资源库,为红豆杉可持续开发提供科学保障。
Abstract:The genetic diversity and population structure of Taxus germplasm resources were analyzed based on 15 ScoT primers with 76 Taxus samples from 11 populations in 5 counties of Hanzhong city,in order to provide theoretical basis for regional protection and effective development and utilization of Taxus wallichiana var. chinensis germplasm resources in Qinling Mountains of Hanzhong. The results showed that:( 1) The genetic diversity of Taxus wallichiana var. chinensis was low at the locus level( Na = 1. 96,Ne = 1. 49,H =0. 30,I = 0. 46).( 2) Cluster analysis divided the tested materials into 4 groups at the locus level,which was basically consistent with the results of population structure analysis( K = 4).( 3) The genetic diversity of Taxus wallichiana var. chinensis population was low( Na = 1. 52,Ne = 1. 30,H = 0. 18,I = 0. 37) and the genetic variation mainly came from within the population( 79%).( 4) The 11 populations of Taxus wallichiana var. chinensis were clustered into 4 groups. The GZ population of Lueyang County and the 4 populations of Liuba County were clustered into one group. The Mianxian population and the 2 populations of Foping County were clustered into one group,which was consistent with the results of principal coordinate analysis( PCoA). Based on the above results,the genetic diversity of Taxus wallichiana var. chinensis in the Qinling Mountains of Hanzhong is relatively low. It is suggested to carry out in-situ protection and build a multi-resource germplasm resource bank for Taxus wallichiana var. chinensis in the Qinling Mountains of Hanzhong,so as to provide scientific guarantee for the sustainable development of Taxus wallichiana var. chinensis.
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基本信息:
中图分类号:S791.49
引用信息:
[1]程文娜,魏丽娜,胡佳,等.SCoT标记分析汉中秦岭地区红豆杉遗传多样性[J].陕西理工大学学报(自然科学版),2025,41(05):18-24+72.
基金信息:
陕西红豆杉国家林木种质资源异地保存建设项目(JZ2021-48)